The following is a document detailing the analysis method to determine the existence of appetite suppressant element P57 on hoodia gordonii plants.

P-068: DETERMINATION OF THE APPETITE SUPPRESSANT P57 IN HOODIA GORDONII PLANT EXTRACTS AND DIETARY SUPPLEMENTS BY LIQUID CHROMATOGRAPHY/ELECTROSPRAY IONIZATION MASS SPECTROMETRY (LC-MSD-TOF) AND LC-UV METHODS

Bharathi Avula1, Yan-Hong Wang 1, Rahul S. Pawar1, Yatin Shukla2, Brian T. Schaneberg3 and Ikhlas A. Khan1

1National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences; 2Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, MS 38677, USA; 3ChromaDex Analytics, Boulder, CO 80301 USA

Hoodia gordonii is traditionally used in S. Africa for its appetite suppressant properties.

P57AS3 (P57), an oxypregnane steroidal glycoside, is the only reported active constituent from this plant as an appetite suppressant.

Effective quality control of these extracts or products requires rapid methods to determine P57 content.

New methods (LC-MS and LC-UV) for analysis of P57 from H. gordonii have been developed.

The quantitative determination of P57 was achieved with a Phenomenex reversed phase C18 column using gradient mobile phase of water and acetonitrile, both containing 0.1% acetic acid.

The method was validated for linearity, repeatability, limits of detection (LOD) and limits of quantification (LOQ). Good results were obtained in terms of repeatability (relative standard deviation (RSD) < 5.0 %) and recovery (98.5%-103.5%).

The developed methods were applied to the determination of P57 for two H. gordonii plant samples, one related Genus (Opuntia ficus-indica) and dietary supplements that claim to contain H. gordonii.